Information4'-Demethylpodophyllotoxin is isolated from the herb of Dysosma pleiantha (Hance) Woodson with remarkable cytotoxic potential in diverse cancer cell lines.In vivoThe mean pharmacokinetic parameters of 4\'-Demethylpodophyllotoxin after oral administration of 367 mg/kg Diphylleia sinensis extract: Cmax=42.4 ± 14.6 ng/ml, Tmax= 1.60 ± 0.92 h, T1/2z=3.35 ± 0.85 h, AUC(0-t)=262 ± 122 ng·h/ml and AUC(0-∞)=301 ± 129 ng·h/ml.