Ponceau S是一种重氮染料的钠盐。它可用于制备染色剂,可用于硝酸纤维素膜或PVDF膜(蛋白质印迹)上蛋白质条带的快速可逆染色,也可用于在醋酸纤维素膜上对蛋白进行染色。可通过水洗涤逆转染色过程,其有助于随后的免疫学检测。以下是两种常见的染色配方: 溶于5%乙酸中的0.1%Ponceau S(w/v) 溶于30%TCA和30%磺基水杨酸中2%Ponceau S(w/v) ;Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection. The following are two common stain formulations: 0.1% Ponceau S (w/v) in 5% acetic acid 2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid