货号	规格	价格	上海	北京	珠海	重庆	武汉
V43709-5mg	98.83%	￥200.00 V43709-5mg ￥200.00 现货	2 2	-	-	-	-
V43709-10mg	98.83%	￥310.00 V43709-10mg ￥310.00 现货	3 3	-	-	-	-
V43709-25mg促销	98.83%	￥760.00 ￥680.00 V43709-25mg ￥680.00 现货	5 5	-	-	-	-
V43709-50mg促销	≥98%	￥1300.00 ￥1170.00 V43709-50mg ￥1170.00 现货	4 4	-	-	-	-

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产品描述：

BODIPY 505/515-8-C3-COOH is a green fluorescing derivative, as a fluorescent dye for imaging lipid droplets in nannochloropsis. BODIPY 505/515-8-C3-COOH can be used for the research of flow cytometric high-throughput screening and cell sorting.

体外研究：

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Cells:
1. Fresh N. oceanica cultures are diluted to ~4×106 cells/ml with ASW and kept at 22℃ prior to any treatment. Incubate the cells according to your normal protocol.
2. BODIPY 505/515 is dissolved in DMSO at 4 mg/ml and diluted with DMSO to different working stock concentrations.
3. Cell suspensions are supplemented with the appropriate BODIPY 505/515 working stock to a specific DMSO concentration between 2 and 10% (v/v) with final BODIPY concentrations between 0.8 and 4 μg/ml.
4. Pure DMSO was used for control treatments. 1 ml of fresh culture was diluted to ~4×106 cells/ml with ASW and stained with 6% DMSO and 1.2 μg/ml BODIPY for 15 min (non-stressed cultures) or with 10% DMSO and 1.6 μg/ml BODIPY for 36 min (stressed cultures).
5. Upon addition of the dye, samples were vortexed for 5 s and then incubated in the dark for 15 min before flow cytometric analysis, if not indicated otherwise.

参考文献：

1. ChristianSüdfeld, et al. Optimization of high-throughput lipid screening of the microalga Nannochloropsis oceanica using BODIPY 505/515. Algal Research,

保存条件：

2-8°C, 避光

配置溶液浓度参考：

	1mg	5mg	10mg
1 mM	2.992 ml	14.962 ml	29.925 ml
5 mM	0.598 ml	2.992 ml	5.985 ml
10 mM	0.299 ml	1.496 ml	2.992 ml
50 mM	0.06 ml	0.299 ml	0.598 ml

注意：

部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

参考文献

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