货号	规格	价格	上海	北京	珠海	重庆	武汉
V45504-500μg	≥98%	￥1220.00 V45504-500μg ￥1220.00 3-5天	0 货期：3-5天	-	-	-	-
V45504-1mg	≥98%	￥2290.00 V45504-1mg ￥2290.00 3-5天	0 货期：3-5天	-	-	-	-
V45504-5mg	≥98%	￥5760.00 V45504-5mg ￥5760.00 3-5天	0 货期：3-5天	-	-	-	-
V45504-10mg	≥98%	￥9210.00 V45504-10mg ￥9210.00 3-5天	0 货期：3-5天	-	-	-	-
V45504-25mg	≥98%	￥16500.00 V45504-25mg ￥16500.00 3-5天	0 货期：3-5天	-	-	-	-

大包装询价收藏产品

加入购物车

到货通知，提交手机号入库后将短信通知。

产品描述：

Ac-ANW-AMC is a fluorogenic substrate for immunoproteasome. Ac-ANW-AMC can be used to measure β5i activity (Ex=345 nm, Em=445 nm).

体外研究：

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. For the proteasome activity assay, cells are lysed in the proteasome activity lysis buffer.
2. Cells are homogenized by passing the lysates 15 times through a 26G×1/2″ needle attached to a 1-mL syringe.
3. The lysates are then centrifuged at 12,000 rpm for 15 min. Protein concentrations of the samples are determined using the Bradford assay.
4. Proteasome activity is determined using model peptide substrates by measuring free Ac-ANW-AMC fluorescence on a TECAN infinite m200 fluorometer.
5. The fluorescence unquenched after hydrolysis by proteasomes is monitored every three minutes at 345 nm excitation and 445nm emission wavelengths at 30°C.
Note: Each sample is assayed in triplicate.

保存条件：

-20°C, 避光

配置溶液浓度参考：

	1mg	5mg	10mg
1 mM	1.699 ml	8.495 ml	16.989 ml
5 mM	0.34 ml	1.699 ml	3.398 ml
10 mM	0.17 ml	0.849 ml	1.699 ml
50 mM	0.034 ml	0.17 ml	0.34 ml

注意：

部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

参考文献

质检证书(COA)

输入产品批号：

如何获取质检证书(COA)?

请输入货号和一个与之匹配的批号。
例如:
批号：JS298415 货号：S20001-25g
在货品标签上如何找到货号和批号?

摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子摩尔量 (g/mol)

质量

浓度

体积

分子摩尔量

计算重置