货号	规格	价格	上海	北京	珠海	重庆	武汉	购买数量
V46517-1mg	≥98%	￥660.00 V46517-1mg ￥660.00 3-5天	0 货期：3-5天	-	-	-	-

货号

规格

价格

上海

北京

珠海

重庆

武汉

购买数量

V46517-1mg

≥98%

￥660.00

货期：3-5天

产品描述：

FBBBE is used to detect the production of H2O2 by cells. FBBBE can be triggered by intracellular H2O2 and converted to fluorescein, resulting in an increase in intracellular fluorescence (Ex=4480 nm, Em=512 nm).

体外研究：

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. The RAW 264.7 cells are seeded at 250,000 in glass bottom 35 mm dishes and post-adhering (5 h).
2. Some dishes are treated with 1 μg/mL of LPS for 24 h. Other dishes are treated the next day after plating with either 100 μM H2O2 for 1 h or PBS for 1 h.
3. Cells are washed twice with PBS and fixed in ice-cold 95% ethanol for 15 min.
3. Cells are twice rinsed with PBS and incubated with either 50 μM probe (FBBBE) for 1 h at room temperature.
4. Cells are washed twice with PBS and coverslips were mounted over cells using Aqua Mount.
5. Confocal images are obtained at 80× magnification on an Olympus FV1000 confocal laser scanning microscope.
6. Images were processed using ImageJ software.

保存条件：

-20°C, 避光, 氮气保存

配置溶液浓度参考：

	1mg	5mg	10mg
1 mM	1.308 ml	6.54 ml	13.081 ml
5 mM	0.262 ml	1.308 ml	2.616 ml
10 mM	0.131 ml	0.654 ml	1.308 ml
50 mM	0.026 ml	0.131 ml	0.262 ml

注意：

部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

参考文献

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