| 产品描述: | PFB-FDG is a non-fluorescent galactosidase substrate that can be hydrolysed to green fluorescent PFB-F (Ex=485 nm, Em=535 nm). PFB-FDG can be used for the determination of β-galactosidase activity. |
| 体外研究: |
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Measurement of β-galactosidase activity: 1. Incubate 2 × 105 cells with 50 μg/mL of PFB-FDG in serum-free DMEM containing 25 mM Hepes (pH 7.4) for 2 h at 37℃. (Mononuclear cells for example, different cell concentrations vary) 2. Wash cells once with ice-cold PBS. 3. Analyze cells by flow cytometry (Ex=485 nm, Em=535 nm). |
| 参考文献: |
1. Navarrete Santos A, et al. Evidences for age-related modulation of human hematopoietic progenitor cell proliferation. Exp Gerontol. 2008 Nov;43(11):1033-8.
|
| 保存条件: |
-20°C, 避光 |
| 配置溶液浓度参考: |
|
1mg |
5mg |
10mg |
| 1 mM |
1.155 ml |
5.776 ml |
11.552 ml |
| 5 mM |
0.231 ml |
1.155 ml |
2.31 ml |
| 10 mM |
0.116 ml |
0.578 ml |
1.155 ml |
| 50 mM |
0.023 ml |
0.116 ml |
0.231 ml |
|
| 注意: |
部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。 |