| 产品描述: | AC-green (VDP-green) is a β-allyl carbamate fluorescent probe for specifically imaging vicinal dithiol proteins (VDPs) in living systems (λex/λem=400/475 nm). AC-green can detect the reduced bovine serum albumin (rBSA) with high sensitivity. AC-green displays low toxicity and features high sensitivity, and is suitable for sensing VDPs in living cells and zebrafishes. |
| 体外研究: |
AC-green (VDP-green) can respond to VDPs with more than 60-fold increase of emission in aqueous solution, while there is no significant interference from biological thiols, amino acids or inorganic salts. AC-green (2 μM; 90 min) has no apparent fluorescence signal within the pH range of 5.0-9.0. Addition of rBSA turns on the fluorescence. AC-gree (10 μM) has low cytotoxicity in HepG2 cells and Hela cells. AC-gree (10 μM; for 15 min) images VDPs in living HepG2 cells and bright green fluorescence appeared. This fluorescence is inhibited when the cells are pretreated with PAO, a popular specificligand for protein vicinal dithiols. AC-gree (10 μM; for 20 min) incubates with zebrafishes has a strong fluorescence signal appeared in the green channel. |
| 保存条件: |
-20°C, 避光 |
| 配置溶液浓度参考: |
|
1mg |
5mg |
10mg |
| 1 mM |
2.296 ml |
11.482 ml |
22.964 ml |
| 5 mM |
0.459 ml |
2.296 ml |
4.593 ml |
| 10 mM |
0.23 ml |
1.148 ml |
2.296 ml |
| 50 mM |
0.046 ml |
0.23 ml |
0.459 ml |
|
| 注意: |
部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。 |