货号	规格	价格	上海	北京	珠海	重庆	武汉	购买数量
V48363-50μg	≥98%	￥1620.00 V48363-50μg ￥1620.00 3-5天	0 货期：3-5天	-	-	-	-

货号

规格

价格

上海

北京

珠海

重庆

武汉

购买数量

V48363-50μg

≥98%

￥1620.00

货期：3-5天

产品描述：

Calcium Orange AM is an intracellular calcium reporter. Specific fluorescence can be detected when free calcium binds to Calcium Orange AM (Ex/Em=549/576 nm). Calcium Orange AM does not enter the vacuoles and does not compartmentalize into acidic vesicles.

体外研究：

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Calcium Orange AM is freshly prepared in dehydrated DMSO before each experiment.
2. Prepare the recording medium for measuring [Ca2+]i in cells consisted of 20 mM HEPES, 115 mM NaCl, 5.4 mM KCl, 0.8 mM MgCl2, 1.8 mM CaCl2, and 13.8 mM D-glucose adjusted to pH 7.4 by adding 1 M KOH.
3. Exchange the culture medium for 1 mL of a recording medium including 10 μM Calcium Orange AM.
4. Incubate cells under conditions of 5% CO2 and 37°C for 40 min.
5. Remove the recording medium, including calcium orange AM from the dish, and add the culture medium after washing it three times with 1 mL of the recording medium.
6. Incubate the cells for 1 h. After incubation, exchange the culture medium for the recording medium again.
7. Detect fluorescence.

保存条件：

-20°C, 避光

配置溶液浓度参考：

	1mg	5mg	10mg
1 mM	0.818 ml	4.088 ml	8.175 ml
5 mM	0.164 ml	0.818 ml	1.635 ml
10 mM	0.082 ml	0.409 ml	0.818 ml
50 mM	0.016 ml	0.082 ml	0.164 ml

注意：

部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

参考文献

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