货号	规格	价格	上海	北京	珠海	重庆	武汉
V48443-1mg	≥90%	￥1250.00 V48443-1mg ￥1250.00 3-5天	0 货期：3-5天	-	-	-	-
V48443-5mg	≥90%	￥2600.00 V48443-5mg ￥2600.00 3-5天	0 货期：3-5天	-	-	-	-
V48443-10mg	≥90%	￥3400.00 V48443-10mg ￥3400.00 3-5天	0 货期：3-5天	-	-	-	-
V48443-25mg	≥90%	￥5100.00 V48443-25mg ￥5100.00 3-5天	0 货期：3-5天	-	-	-	-
V48443-50mg	≥90%	￥8140.00 V48443-50mg ￥8140.00 3-5天	0 货期：3-5天	-	-	-	-
V48443-100mg	≥90%	￥13000.00 V48443-100mg ￥13000.00 3-5天	0 货期：3-5天	-	-	-	-

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产品描述：

Cy3 methyltetrazine (TZ-Cy3) is a click chemistry reagent with methyltetrazine building blocks that is highly reactive towards cyclooctene.
Cy3 methyltetrazine is also a tetrazine-modified fluorescent probe that can be used to analyze protein phosphorylation in solution and living cells.

体外研究：

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Confocal imaging of phosphorylation in cells:
1. Culture H1299 cells in a coverglass-bottom imaging dish for 24 h (take H1299 cells as an example).
2. Incubate H1299 cells with different concentrations of ATP-NB (a ATP analogue functionalized by norbornene, which can penetrate cells and efficiently phosphorylate proteins in living cells) for 1 h.
3. Add Cy3 methyltetrazine to label the phosphorylated protein with fluorescence.
4. Incubate with fresh medium for 1 h.
5. Wash the cells twice with PBS to release the free Cy3 methyltetrazine probe.
6.Incubate the cells with 4% paraformaldehyde for 15 min and with 0.1% Triton X-100 solution at room temperature for 10 min.
7. Wash cells 4 times with PBS.
8. Use a confocal microscopy imaging system to obtain the high-resolution images of cells (Ex=488 nm; Em=561 nm).

保存条件：

-20°C,干燥, 避光

配置溶液浓度参考：

	1mg	5mg	10mg
1 mM	1.374 ml	6.872 ml	13.743 ml
5 mM	0.275 ml	1.374 ml	2.749 ml
10 mM	0.137 ml	0.687 ml	1.374 ml
50 mM	0.027 ml	0.137 ml	0.275 ml

注意：

部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

参考文献

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