Liver-to-body weight ratio is attenuated by Betaine hydrochloride supplementation. When Betaine hydrochloride is added to the alcohol-containing liquid diet, both hepatic triglyceride (TG) accumulation and liver injury are significantly reduced. Betaine hydrochloride supplementation attenuates epididymal fat pad mass loss induced by chronic alcohol feeding. Betaine hydrochloride supplementation prevents the depletion of S-adenosylhomocysteine (SAM) and reduces the increase in S-adenosylhomocysteine (SAH), thereby improving the methylation state of the adipose tissue.