| 产品描述： | Sortin1 is a chemical genetic-hit molecule that causes specific mislocalization of plant and yeast-soluble and membrane vacuolar markers. |
| 体外研究： |
Sortin1 was found to redirect the vacuolar destination of plant carboxypeptidase Y and other proteins in Arabidopsis suspension cells and cause these proteins to be secreted. In Arabidopsis seedlings, the application of Sortin1 and -2 led to reversible defects in vacuole biogenesis and root development. Sortin1 treatment of whole Arabidopsis seedlings also resulted in carboxypeptidase Y secretion, indicating that the drug has a similar mode of action in cells and intact plants. Structure-activity relationship studies conducted in Arabidopsis revealed the structural requirements for Sortin1 bioactivity and demonstrated that overlapping Sortin1 substructures can be used to discriminate between vacuolar-flavonoid accumulations and vacuolar-biogenesis defects |
| 参考文献： |
1. Zouhar J, et al. Sorting inhibitors (Sortins): Chemical compounds to study vacuolar sorting in Arabidopsis. Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9497-501. 2. Orr DJ, et al. 1H NMR-based metabolomics methods for chemical genomics experiments. Methods Mol Biol. 2014;1056:225-39. 3. Rosado A, et al. Sortin1-hypersensitive mutants link vacuolar-trafficking defects and flavonoid metabolism in Arabidopsis vegetative tissues. Chem Biol. 2011 Feb 25;18(2):187-97. |
| 溶解性： |
Soluble in DMSO |
| 保存条件： |
-80℃（运输条件：冰袋低温运输） |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.265 ml |
11.327 ml |
22.654 ml |
| 5 mM |
0.453 ml |
2.265 ml |
4.531 ml |
| 10 mM |
0.227 ml |
1.133 ml |
2.265 ml |
| 50 mM |
0.045 ml |
0.227 ml |
0.453 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
北京