| 产品描述： | PolQi1 is a selective inhibitor targeting the Polθ domain of DNA polymerase. PolQi1 inhibits the Polθ-mediated microhomology end joining (TMEJ/alt-EJ) pathway, reducing insertion/deletion (Indels) and imprecise editing events during DNA repair. PolQi1 can enhance the efficiency and accuracy of homology-directed repair (HDR) or Prime editing, and reduce off-target effects; and in combination with DNA-PK inhibitor AZD-7648 , exert efficient genome editing capabilities with dual pathway regulation. PolQi1 can be mainly used in gene editing research (such as CRISPR-Cas9 or Prime editing system optimization) to improve the precision editing efficiency of difficult-to-edit cells (such as primary hepatocytes and mouse embryos). |
| 体内研究： |
PolQi1 (3 μM；3 h) 与 AZD7648 联用时，可提高 HEK293T 和人诱导多能干细胞 (hiPSCs) 中的同源定向修复 (HDR) 介导的基因敲入效率并减少插入/缺失 (InDels)。 |
| 保存条件： |
-80°C 6个月;-20°C 1个月 |
| 配置溶液浓度参考： |
|
1mg |
5mg |
10mg |
| 1 mM |
2.228 ml |
11.142 ml |
22.283 ml |
| 5 mM |
0.446 ml |
2.228 ml |
4.457 ml |
| 10 mM |
0.223 ml |
1.114 ml |
2.228 ml |
| 50 mM |
0.045 ml |
0.223 ml |
0.446 ml |
|
| 注意： |
部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。 |
上海工商
危险品化学品经营许可证（不带存储）
营业执照(三证合一)
