| 产品描述: | Cas9-IN-1 is a potent Cas9 inhibitor (IC50=7.02 μM), acting by binding to apo-Cas9 to prevent Cas9:gRNA complex formation. |
| 体内研究: |
Cas9-IN-1 (compound 85) (5 and 13 μM; overnight) can detach gRNA from preformed Cas9:gRNA complex while having weaker inhibitory activity, helping the E. coli selection strain to survive SpyCas9-induced killing. |
| 参考文献: |
1. Sang-Woo Lee et al. Identification and Optimization of Novel Small-Molecule Cas9 Inhibitors by Cell-Based High-Throughput Screening. Journal of Medicinal Chemistry 2022; 65(4), 3266-3305. |
| 保存条件: |
-80°C 6个月;-20°C 1个月 |
| 配置溶液浓度参考: |
|
1mg |
5mg |
10mg |
| 1 mM |
2.216 ml |
11.08 ml |
22.161 ml |
| 5 mM |
0.443 ml |
2.216 ml |
4.432 ml |
| 10 mM |
0.222 ml |
1.108 ml |
2.216 ml |
| 50 mM |
0.044 ml |
0.222 ml |
0.443 ml |
|
| 注意: |
部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。 |