| 产品描述: | Msr-blue is a first turn-on fluorescent probe for methionine sulfoxide reductase with a more than 100-fold fluorescence increment. Msr-blue is used for monitoring the enzyme activity in live cells (λex=340 nm, λem=440 nm). |
| 体内研究: |
Msr-blue is emited blue fluorescence after activation by methionine sulfoxide reductase A (Msr A). Msr-blue responded to Msr A in both a time- and dose-dependent manner, and more than a 100-fold increase in the emission is observed. Msr-blue is converted to its corresponding sulfide (15′) under catalysis by either the purified Msr A or a cell lysate. The 6-OHDA-treated PC12 cells as a cellular model of Parkinson's disease (PD) is employed and applied Msr-blue to probe the function of Msrs in the cells. With the aid of Msr-blue, a decline of the Msr activity in a PD model was disclosed for the first time. |
| 保存条件: |
-80°C 6个月; -20°C 1个月,避光 |
| 配置溶液浓度参考: |
|
1mg |
5mg |
10mg |
| 1 mM |
4.499 ml |
22.496 ml |
44.992 ml |
| 5 mM |
0.9 ml |
4.499 ml |
8.998 ml |
| 10 mM |
0.45 ml |
2.25 ml |
4.499 ml |
| 50 mM |
0.09 ml |
0.45 ml |
0.9 ml |
|
| 注意: |
部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。 |