货号	规格	价格	上海	北京	珠海	重庆	武汉
S87825-10mg买3赠1	98.40%	￥570.00 S87825-10mg ￥570.00 现货（买3赠1）	5 5	-	-	-	-
S87825-50mg	98.40%	￥1650.00 S87825-50mg ￥1650.00 现货	3 3	-	-	-	-
S87825-100mg	≥98%	￥2950.00 S87825-100mg ￥2950.00 2-3天	0 货期：2-3天	-	-	-	-

货号

规格

价格

上海

北京

珠海

重庆

武汉

购买数量

S87825-10mg买3赠1

98.40%

￥570.00

S87825-50mg

98.40%

￥1650.00

S87825-100mg

≥98%

￥2950.00

货期：2-3天

产品描述：

DLin-KC2-DMA is an ionisable cationic lipid (pKa≈6) that is virtually non-toxic to antigen presenting cells (APCs). DLin-KC2-DMA produces significant siRNA-mediated gene silencing of GAPDH, when binds to lipid nanoparticles (LNP). DLin-KC2-DMA can be used in siRNA delivery studies

靶点：

Liposome

体外研究：

DLin-KC2-DMA (1, 5 μg; 72 h) effectively produces a significant siRNA-mediated GAPDH gene silencing in both macrophages and dendritic cells. DLin-KC2-DMA (24 h) exhibits high uptake into macrophages and dendritic cells. DLin-KC2-DMA efficiently promotes release of encapsulated siRNA into the cytosol following uptake via the endocytotic pathway. DLin-KC2-DMA displays almost no toxicity in primary APCs. Cell Cytotoxicity Assay Cell Line: Macrophages, dendritic cells Concentration: 5 μg/mL (DLin-KC2-DMA contained-LNPs) Incubation Time: 72 h Result: Displayed almost no toxicity. Western Blot Analysis Cell Line: Macrophages, dendritic cells Concentration: 1, 5 μg Incubation Time: 72 h Result: Exhibited significant GAPDH silencing of more than 60% at 1 μg and of 80% at 5 μg in macrophages.Significantly reduced GAPDH protein and exhibited the silencing effects of 83% at 5 μg.

体内研究：

DLin-KC2-DMA contained-LNP siRNA systems (5 mg/kg; i.v.; single) effectively silences target genes in APcs in vivo. Animal Model: Naive C57BL/6 mice. Dosage: 3 or 5 mg/kg (DLin-KC2-DMA contained-LNPs) Administration: Intravenous injection; single. Result: Significantly reduced GAPDH production in peritoneal cavity macrophages and dendritic cells and in the spleen-derived APCs when at 5 mg/kg.

参考文献：

1. Basha G, et al. Influence of cationic lipid composition on gene silencing properties of lipid nanoparticle formulations of siRNA in antigen-presenting cells. Mol Ther. 2011 Dec;19(12):2186-200. 2. Miller AD, et al. Delivery of RNAi therapeutics: work in progress. Expert Rev Med Devices. 2013 Nov;10(6):781-811.

溶解性：

Soluble in DMSO、Ethanol

保存条件：

-20℃，充氮保存

配置溶液浓度参考：

	1mg	5mg	10mg
1 mM	1.557 ml	7.787 ml	15.574 ml
5 mM	0.311 ml	1.557 ml	3.115 ml
10 mM	0.156 ml	0.779 ml	1.557 ml
50 mM	0.031 ml	0.156 ml	0.311 ml

注意：

部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，仅供客户参考交流研究之用。

参考文献

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