PE-Cy5 is a tandem fluorophore comprising R-Phycoerythin and a red-shifted cyanine dye. The resonance energy transfer dye Cy5-Phycoerythrin (Cy5PE) is an extremely bright dye, primarily because of the very low autofluorescence in the region where it fluoresces. It is easily excited at 488 nm, and emits at 680 nm; however, it is also well excited at 633 nm (HeNe laser), and thus using Cy5PE reagents in two laser-system requires interlaser compensation.Many applications and instruments were developed specifically for PE-Cy5. It is commonly used in immunoassays such as FACS, flow cytometry, multimer/tetramer applications. With new instrumentation available, PE-Cy5 is also well suited for immunohistochemistry (IHC) and Western blot applications.