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罗丹明123

    
生物技术级,98%(HPLC)

Rhodamine 123

源叶
S19123 一键复制产品信息
62669-70-9
C21H17ClN2O3
380.82
MFCD00012664
货号 规格 价格 上海 北京 珠海 重庆 武汉 购买数量
S19123-5mg
生物技术级,98%(HPLC)

¥140.00

货期:2-3天 >10 4 - 2
S19123-25mg
生物技术级,98%(HPLC)

¥290.00

3 1 - - 2
S19123-100mg
生物技术级,98%(HPLC)

¥890.00

1 1 - - -
S19123-500mg
生物技术级,98%(HPLC)

¥3200.00

货期:2-3天 - - - -
S19123-1g
生物技术级,98%(HPLC)

¥5600.00

货期:2-3天 - - - -
产品介绍 参考文献(41篇) 质检证书(COA) 摩尔浓度计算器 相关产品

产品介绍

罗丹明123 (Rhodamine 123、罗丹明123, Rh 123)是一种常用的阳离子绿色荧光染料,可以透过细胞膜从而在活细胞线粒体内聚集,并发出黄绿色荧光。广泛用作检测线粒体膜电位的荧光探针,并且对细胞没有任何毒性。 

溶液的配制

用DMSO溶解,终浓度为1-5 mM。溶液分装,避光保存于-20 ºC。

罗丹明123使用方法

1. 用载玻片准备细胞。细胞数目应为 5×104~5×105个/mL。

2. 孵育该载玻片,用 PBS或Hank’s 液洗涤细胞。

3. 用培养基稀释 Rh123母液以制备 1~20µM Rh123缓冲液。具体工作浓度取决于细胞类型和细胞浓度。

4. 将 Rh123缓冲液加入载玻片并在37℃下孵育 30 分钟至 1 小时。

5. 去除 Rh123 缓冲液并用培养基洗涤细胞(洗涤细胞后为了固定,加入 10%福尔马林缓冲液并孵育 15~20 分钟,接着用 PBS洗涤)。

6. 用带有荧光素滤光片的荧光显微镜观察细胞。

为了您的安全和健康,请穿实验服并戴一次性手套操作。

SMILES: O=C(C1=CC=CC=C1C2=C3C=CC(N)=CC3=[O+]C4=C2C=CC(N)=C4)OC.[Cl-]
熔点: 235℃
溶解性: Soluble  in  ethanol  (20  mg/ml),  DMSO,  methanol,  and  water  (partly  miscible).
储存条件: RT
注意: 部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。

参考文献(41篇)

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[IF=6.5] Yinghui Shang et al."Biomimetic Nanoplatform Based on the Neutrophil Membrane for Targeted Therapy of Spinal Cord Injury."International Journal of Nanomedicine. 31. [IF=6.2] Chenyan Zhou et al."Mitochondrial AOX Activation Drives Pyribencarb Resistance in Sclerotinia sclerotiorum: Cellular Trade-Offs and Nontarget Site Mechanisms."JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY.2025;XXXX(XXX):XXX-XXX 30. [IF=1.4] Min-Min Cao et al."Polycyclic polyprenylated acylphloroglucinols with cytotoxicity against human tongue squamous carcinoma cells in vitro from Hypericum curvisepalum."Phytochemistry Letters.2025 Oct;69:103038 29. [IF=1.4] Min-Min Cao et al."Polycyclic polyprenylated acylphloroglucinols with cytotoxicity against human tongue squamous carcinoma cells in vitro from Hypericum curvisepalum."Phytochemistry Letters.2025 Oct;69:103038 28. 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[IF=3.2] Zhiqing Chen et al."Intermittent fasting applied in combination with astaxanthin alleviates D-galactose-induced aging in rats: Comparison in oxidative stress, immune response, and metabolomics."JOURNAL OF FOOD SCIENCE.2025 Apr;90(4):e70170 23. [IF=2.5] Hu Jiudong et al."Tenacissoside G reverses paclitaxel resistance by inhibiting Src/PTN/P-gp signaling axis activation in ovarian cancer cells."Journal of Natural Medicines.2025 Apr;:1-18 22. [IF=3.2] Ziqin Wei et al."Regulatory mechanism of ABCB1 transcriptional repression by HDAC5 in rat hepatocytes under hypoxic environment."Frontiers in Physiology.2025 Apr;16:1520246 21. [IF=8.2] Zhen Cheng et al."Construction of nanoparticles from blueberry anthocyanins-lecithin/gum Arabic improves lipid droplet accumulation and gut microbiota disturbance in HFD-induced obese mice."INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES".2024 Apr;264: 20. 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