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蛋白酶K

    
30u/mg

Proteinase K

源叶
S10085 一键复制产品信息
39450-01-6
MFCD00132129
货号 规格 价格 上海 北京 珠海 重庆 武汉 购买数量
S10085-100mg促销
30u/mg

¥300.00 ¥255.00

>10 >10 6 - 2
S10085-500mg促销
30u/mg

¥1000.00 ¥840.00

>10 - - - -
S10085-1g促销
30u/mg

¥1500.00 ¥1350.00

>10 - - - -
产品介绍 参考文献(30篇) 质检证书(COA) 摩尔浓度计算器 相关产品

产品介绍

本产品来源于表达经过定点突变优化的林伯氏白色念球菌(Engyodontium album)蛋白酶 K 基因的毕赤酵母,具有比活性高、产量高及更广的温度/pH 值活性范围等优势。酵母高效表达和色谱纯化保证了产品的纯度,应用范围比天然蛋白酶 K 更广泛。在 SDS、尿素或EDTA 存在的情况下依旧保持活性。蛋白酶 K 切割脂肪族氨基酸和芳香族氨基酸的羧基端肽键。

活力定义:在 37℃,pH 7.5 的条件下,每分钟释放 1 µM 酪氨酸所需要的 Proteinase K 量定义为 1 个 活性单位。

PI:8.9

E.C.:3.4.21.64

分子量:29.3 kDa

最适 pH:pH7.5-8.0(工作范围 pH4.0-12.5)

最适温度:50-55℃(工作范围 0-75℃)

 

溶解性: 推荐溶解缓冲液:20mM  Tris-HCl(pH  7.4),  1  mM  CaCl2或  20mM  Tris-HCl(pH  7.4),  1  mM  CaCl2,  2%甘油
储存条件: -20℃
EC编号: 3.4.21.64
用途: 分离DNA和RNA期间使核苷酸酶蛋白失活。 去除内毒素对阳离子蛋白的约束,如溶菌酶和核糖核酸酶。 研究报告显示用于分离肝,酵母,绿豆线粒体 测定细胞膜上的酶 用于抗体标记,石蜡包埋组织切片中暴露抗原结合位点。 消化脑组织样本中的蛋白质,用于朊病毒的传染性海绵状脑病(TSE)的研究
注意: 部分产品我司仅能提供部分信息,我司不保证所提供信息的权威性,仅供客户参考交流研究之用。

参考文献(30篇)

25. [IF=5.9] Ziwei Wang et al."Enhanced accumulation of organic selenium and amylopectin in wheat grains: Insights into selenite-driven biotransformation processes."Food Bioscience.2026 Feb;76:108246 24. [IF=5.9] Zhaoyi Zhang et al."Distinct mechanisms of N-glycolylneuraminic acid reduction by Lactiplantibacillus plantarum R2 and Staphylococcus carnosus C1: Adsorption versus biodegradation."Food Bioscience.2025 Dec;74:107937 23. [IF=5.9] Haousu Zhang et al."A novel Bacillus paramycoides HS-1 Capable of Degrading Aflatoxin B1 and Its Key Enzymatic Mechanisms."Food Bioscience.2025 Jun;:107039 22. [IF=10.7] Bingqing Jia et al."Deciphering the Physical Binding Mechanism of Enzyme–Photosensitizer Facilitates Catalysis-Augmented Photodynamic Therapy."Research.2025 Jun;8 21. [IF=6.5] Feiran An et al."Effects of exogenous selenium application on quality characteristics, selenium speciation, and in vitro bioaccessibility of rice pancakes."Food Chemistry-X.2024 Dec;:102064 20. [IF=3.5] Wei Dong et al."PTX3-assembled pericellular hyaluronan matrix enhances endochondral ossification during fracture healing and heterotopic ossification."BONE.2025 Mar;192:117385 19. [IF=4] Meng Fanxing et al."Study on molecular characteristics of Staphylococcus from yak milk-Xizang."BMC MICROBIOLOGY.2025 Dec;25(1):1-16 18. [IF=3.5] Jiawen Shen et al."Rhizoma Drynariae-derived EV-like particles alleviate osteoporosis by promoting osteogenic differentiation in BMSCs through the activation of the hsa_circ_0001275/miR-422a pathway."BONE.2025 Apr;:117489 17. [IF=7.7] Yuxia Xie et al."Preparation, characterization, and mechanism of DPP-IV inhibitory peptides derived from Bactrian camel milk."INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES.2024 Oct;277:134232 16. [IF=8.5] Furong Wang et al."Accumulation and metabolism of selenium in the rare yeast Kazachstania unispora during the selenium enrichment process."FOOD CHEMISTRY.2024 Nov;459:140375 15. [IF=4.9] Huang Tian et al."Preliminary Purification and Partial Characterization of a Functional Bacteriocin of Lacticaseibacillus paracasei Zhang and Mining for its Gene Cluster."Probiotics and Antimicrobial Proteins".2024 May;:1-13 14. [IF=8.1] Shuojia Liang et al."In vitro digestion and fecal fermentation of selenocompounds: impact on gut microbiota, antioxidant activity, and short-chain fatty acids."FOOD RESEARCH INTERNATIONAL".2024 Mar;180:114089 13. [IF=15.1] Meng Huang et al."Multi-enzyme mimetic iridium nanozymes-based thrombus microenvironment-modulated nanoplatform for enhanced thrombolytic therapy."CHEMICAL ENGINEERING JOURNAL.2023 Aug;470:144156 12. [IF=8.2] Ren Wang et al."Impact of crystalline structure on the digestibility of amylopectin-based starch-lipid complexes."INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES.2023 Jul;242:125191 11. [IF=4.047] Liu Lu et al."Identification and characterisation of the haemozoin of Haemonchus contortus."Parasites & Vectors.2023 Dec;16(1):1-9 10. [IF=4.098] Tao Liu et al."Rational design of a fluorescent probe for the detection of LAP and its application in drug-induced liver injury."Spectrochim Acta A. 2021 Apr;251:119362 9. [IF=4.952] Li Li et al."The adhesion of the gut microbiota to insoluble dietary fiber from soy hulls promoted the proliferation of probiotics in vitro."Lwt Food Sci Technol. 2022 Jan;153:112560 8. [IF=5.833] Chen Jing et al."A composite prepared from MnO2 nanosheets and a deep eutectic solvent as an oxidase mimic for the colorimetric determination of DNA."Microchim Acta. 2020 Jan;187(1):1-7 7. Guoqiang Cui, Xuebing Yang, Tingting Liu, Zaizhi Liu, Lei Yang, An efficient approach for the enzyme-enhanced extraction of camptothecin and 10-hydroxycamptothecin from the samara of Camptotheca acuminata using an ionic liquid solution, Separation and Puri 6. 江宇航,李宏伟,杨晓洁,林连兵,张棋麟.马尾松毛虫肠道产细菌素细菌的筛选及抑菌特性[J].微生物学通报,2021,48(01):123-134. 5. 孙小涵,孙洪浩,陈秀秀,吕福军.具有抑菌活性植物乳杆菌的筛选及抑菌物质的特性分析[J].养猪,2020(06):18-20. 4. 高兆建,王秋芬,胡鑫强,宋玉林,赵宜峰,陈腾.凝结芽孢杆菌XZQ-16抗菌脂肽分离鉴定及抗菌特性[J].食品工业科技,2021,42(03):36-42. 3. 高兆建,张艳秋,宋玉林,赵宜峰.筛选自泡菜的发酵乳杆菌细菌素纯化及抑菌特性分析[J].食品工业科技,2021,42(03):201-207+283. 2. 常志远, 徐美玲, 文婷婷,等. 转基因食物中常见外源基因PCR检测方法的建立[J]. 长春理工大学学报(自然科学版), 2019, 042(002):137-142. 1. 阴佳璐, 唐语谦, 任杰,等. 浑浊红球菌PD630对黄曲霉毒素B1的生物降解特性研究[J]. 中国食品添加剂, 2020, 031(002):39-46.

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摩尔浓度计算器

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